5′-Internal & 3′-Amino Modifiers

5′-Amino modifiers are phosphoramidites which are used to produce a functional amine group on the 5′-terminus of oligonucleotides. The modifiers incorporate a C6 spacer with a primary amino group which can be used for subsequent conjugation to a vast majority of applications, such as dyes or labesls. The MMT protecting group can be removed on the DNA synthesizer by DCA treatment (usually 3% DCA/DCM). For cartridge purification, the final cleavage of the MMT group can be accomplished using a TFA solution (usually 2.5% TFA).

• Molecular Formula:  C₃₅H₄₈N₃O₃P

• Molecular Weight: 589.76 g/mol
• Specification:
  • HPLC – Identity is confirmed by comparison with a reference sample, Minimum purity
  • NMR – Purity is determined by ³¹P NMR and isomeric purity is veified by ¹H NMR
  • Coupling Test – Coupling efficiency > 98%
  • Solution Test – 0.1M solution clear and free of particulate contamination
  • Loss on drying – volatile components are determined to be < 2%
  • Water content – determined to be < 0.4%
  • Certificate of Analysis are available for every batch, even for lon past delivered products.