4LSC3226 5′-Amino-Modifier C6 TFA CAS# 133975-85-6

Catalog Number: 4LSC3226
CAS #: 133975-85-6
Name: 5′-Amino-Modifier C6 TFA; 2-cyanoethyl (6-(2,2,2-trifluoroacetamido)hexyl) diisopropylphosphoramidite

5′-Internal & 3′-Amino Modifiers

5′-Amino modifiers are phosphoramidites which are used to produce a functional amine group on the 5′-terminus of oligonucleotides. The modifiers incorporate a C6 spacer with a primary amino group which can be used for subsequent conjugation to a vast majority of applications, such as dyes or labesls. The MMT protecting group can be removed on the DNA synthesizer by DCA treatment (usually 3% DCA/DCM). For cartridge purification, the final cleavage of the MMT group can be accomplished using a TFA solution (usually 2.5% TFA).

  • Molecular Formula:  C17H31F3N3O3P

  • Molecular Weight: 413.42 g/mol
  • Specification:
    • HPLC – Identity is confirmed by comparison with a reference sample, Minimum purity
    • NMR – Purity is determined by 31P NMR and isomeric purity is veified by 1H NMR
    • Coupling Test – Coupling efficiency > 98%
    • Solution Test – 0.1M solution clear and free of particulate contamination
    • Loss on drying – volatile components are determined to be < 2%
    • Water content – determined to be < 0.4%
    • Certificate of Analysis are available for every batch, even for lon past delivered products.