4LSC3235 5′-Phosphate Amidite CAS# 108783-02-4
Catalog Number: 4LSC3235
CAS #: 108783-02-4
Name: 5′-Phosphate Amidite; 2-[2-(4,4′-Dimethoxytrityloxy)ethylsulfonyl]ethyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Typically in stock
5′- & 3′- Phosphorylating Modifiers
5′-Phosphorylating Modifiers are phosphoramidites used as an alternative to enzymatic techniques for 5’phosphorylation of oligonucleotides. The DMT group allows determination of phosphorylation efficiency. The DMT-2,2′-Sulfonyldiethanol group of the 5’Phosphate Amidite is eliminated during the standard ammonium hydroxide treatment, rendering this compound incompatible with the DMT-on reversed-phase purification.
On the other side, the DMT of the 5′-Phosphorylating reagent II is stable to the standard ammonium hydroxide deprotection allowing DMT-on reversed-phase purification. A fnal deblocking of the oligonucleotide can be accomplished with trifluoroacetic acid or with acetic acid / water treatment.
Enquiry about 4LSC3235 5'-Phosphate Amidite CAS# 108783-02-4
We're ready to solve any rare chemicals sourcing or molecular design problem... Give us a shout ! Please fill out this form completely (incomplete form will not be considered).
If you don't find a product in our current offer, please don't hesitate to request a quote using our contact form.