5′- & 3′- Phosphorylating Modifiers

5′-Phosphorylating Modifiers are phosphoramidites used as an alternative to enzymatic techniques for 5’phosphorylation of oligonucleotides. The DMT group allows determination of phosphorylation efficiency. The DMT-2,2′-Sulfonyldiethanol group of the 5’Phosphate Amidite is eliminated during the standard ammonium hydroxide treatment, rendering this compound incompatible with the DMT-on reversed-phase purification.

On the other side, the DMT of the 5′-Phosphorylating reagent II is stable to the standard ammonium hydroxide deprotection allowing DMT-on reversed-phase purification. A fnal deblocking of the oligonucleotide can be accomplished with trifluoroacetic acid or with acetic acid / water treatment.

• Molecular Formula:  C₃₉H₅₁N₂O₉P

• Molecular Weight: 722.82 g/mol
• Specification:
  • HPLC – Identity is confirmed by comparison with a reference sample, Minimum purity
  • NMR – Purity is determined by ³¹P NMR and isomeric purity is veified by ¹H NMR
  • Coupling Test – Coupling efficiency > 98%
  • Solution Test – 0.1M solution clear and free of particulate contamination
  • Loss on drying – volatile components are determined to be < 2%
  • Water content – determined to be < 0.4%
  • Certificate of Analysis are available for every batch, even for lon past delivered products.