4LSC3240 Biotin dT CAS# 198080-40-9
Catalog Number: 4LSC3240
CAS #: 198080-40-9
Name: Biotin dT; 5′-Dimethoxytrityloxy-5-[N-((4-tert-butylbenzoyl)-biotinyl)-aminohexyl)-3-acrylimido]-2′-deoxyUridine-3′-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite
5′ & Internal – Biotin Labeling
Biotin amidites are used in the 5′ labeling of oligonucleotides. Biotin labeling can be captured by avidin or streptavidin. The biotin phosphoramidite is a good alternative to the use of a biotin-NHS ester procedure. This compound allows multiple additons at the 5′-terminus of an oligonucleotide. An improvement in the use of multiple labeling has been achieved through the Biotin-TEG phosphoramidite. The mixed polarity of the triethyleneglycol linker is very useful for multiple additions as well as optimal capture by avidin and streptavidin.
Molecular Formula: C69H89N8O12PS
- Molecular Weight: 1 285.6 g/mol
- HPLC – Identity is confirmed by comparison with a reference sample, Minimum purity
- NMR – Purity is determined by 31P NMR and isomeric purity is veified by 1H NMR
- Coupling Test – Coupling efficiency > 98%
- Solution Test – 0.1M solution clear and free of particulate contamination
- Loss on drying – volatile components are determined to be < 2%
- Water content – determined to be < 0.4%
- Certificate of Analysis are available for every batch, even for lon past delivered products.
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