5′ & Internal – Biotin Labeling

Biotin amidites are used in the 5′ labeling of oligonucleotides. Biotin labeling can be captured by avidin or streptavidin. The biotin phosphoramidite is a good alternative to the use of a biotin-NHS ester procedure. This compound allows multiple additons at the 5′-terminus of an oligonucleotide. An improvement in the use of multiple labeling has been achieved through the Biotin-TEG phosphoramidite. The mixed polarity of the triethyleneglycol linker is very useful for multiple additions as well as optimal capture by avidin and streptavidin.

• Molecular Formula:  C₅₂H₇₆N₅O₁₁PS

• Molecular Weight: 1 010.24 g/mol
• Specification:
  • HPLC – Identity is confirmed by comparison with a reference sample, Minimum purity
  • NMR – Purity is determined by ³¹P NMR and isomeric purity is veified by ¹H NMR
  • Coupling Test – Coupling efficiency > 98%
  • Solution Test – 0.1M solution clear and free of particulate contamination
  • Loss on drying – volatile components are determined to be < 2%
  • Water content – determined to be < 0.4%
  • Certificate of Analysis are available for every batch, even for lon past delivered products.